Enlight  Fluorographic Enhancer

Enlight is a non-toxic aqueous solution that allows the fluorographic enhancement of low-energy isotopes in acrylamide and agarose gels. After electrophoresis the gel is soaked in the Enlight solution for one hour. The gel is then dried as usual and exposed to an x-ray film at -70°C. Low-energy electrons that can not leave the gel matrix (³H!) are likely to interact with fluorographic molecules that are now incorporated in the gel and stimulate a light-emitting process. These photons can easily leave the gel to expose the film. The increased detection efficiency for ³H is at least 10000 fold, for ¹⁴C, ³⁵S and ³³P at least 100 fold. Hence Enlight works better than other known products on the market! Saves time and money!

Article	Quantity	Catalog No.	Price
Enlight	0.5 Lit.	R001 0500	EURO 59.00
Enlight	1 Lit.	R001 1000	EURO 99.00

  14C-labeled protein marker and 
  3H- and 35S-labeled cell lysates
  were separated on a 12% SDS gel, 
  then dried directly or first 
  incubated in Enlight solution
  for one hour befor drying.
  Both gels were exposed 
  to film for 40 h @ -70°C.  
  

Silver Surfer  Single Solution Silver Stain

Silver Surfer combines the sensitivity of conventional silver staining with the speed and ease you've always been waiting for (see your bands 30 minutes after fixing). Whereas old protocols required multiple incubation, washing and development steps, Silver Surfer does it all in one single solution! Moreover contrast and sensitivity have been markedly improved in this kit (down to 200 pg!). While you got brownish bands on your gels until now, Silver Surfer generates a deep black staining color. The silver stain develops over a period of 10 - 20 minutes, to give you enough time to stop the reaction at the point of staining intensity you require. The reaction is stopped in standard fixing solution and gels can be dried and stored as usual. Silver Surfer is suitable for the staining of 2D and DNA gels as well. One kit is enough for 40 SDS mini gels.

Article	Quantity	Catalog No.	Price
Silver Surfer	40 mini gels	W003 0040	EURO 119.00







  Example of a Silver Surfer 
  stained SDS PAGE gel.
  

Snow White  Reversible Zinc Stain

Snow White is a reversible, rapid negative stain for SDS PAGE gels. A simple two step staining procedure allows visualization of bands in just 10 minutes with a sensitivity equal to that of silver stain. Snow White produces an opaque white background with crystal clear protein bands that can easily be viewed and documented when placed against a dark background (e.g. UV screen). The destaining solution of the kit allows complete destaining of the gel in just 15 minutes. Gels can then be applied to subsequent procedures such as Western blotting, electroelution, amino acid analysis, N-terminal sequencing, MALDI-TOF... you name it! One kit stains 25 mini gels.

Article	Quantity	Catalog No.	Price
Snow White	25 mini gels	W002 0025	EURO 79.00

  Example of a Snow White
  stained SDS PAGE gel placed
  against the dark background
  of a UV transilluminator.
  

Deep Blue  Colloidal Coomassie

Deep Blue is the easiest way to Coomassie stain your protein gels. It's sensitive, it doesn't smell, it's fast, and it's destain free! The equilibrium of the blue dye in Deep Blue is primarily driven towards its solid colloid state, leaving the solution almost colorless. Proteins bands inside a gel accumulate these trace amounts of dye quickly and develop a dark blue stain while the rest of the gel remains clear. Watch your bands develop before your eyes, directly in the staining tray. After one hour the staining is complete and the gel can be dried directly. It is also possible to further increase the sensitivity of Deep Blue by an additional water wash step to detect even the weakest bands. Deep Blue offers the best results for the best price!

Article	Quantity	Catalog No.	Price
Deep Blue	0.5 Lit.	W004 0500	EURO 46.00
Deep Blue	1.0 Lit.	W004 1000	EURO 81.00







  Two identical SDS gels stained with
  A) Deep Blue
  B) Conventional Coomassie + destain
  

Bradford  Protein Quantification

The Bradford assay is a widely used and reliable method to determine protein concentrations in solutions in the range of 2 to 300 µg per assay (10 - 1500 µg/ml). It is easy to perform, rapid and very specific for proteins. We now offer this dye together with a protocol for increased linearity of OD to concentration ratio. The kit contains 500 ml of dye reagent, sufficient for 2500 assays in 1 ml cuvettes.

pEG-His1  His-Tagged Cloning Vector

The pEG-His1 vector was particularly constructed for the expression of toxic gene products in E. coli. To obtain its exceptional tightness prior induction with IPTG the Lac I repressor gene has been included in the vector and is overexpressed in plasmid bearing cells.

Features of pEG-His1 are:

	An optimized promoter guarantees excellent expression levels.
	Very tight expression control due to overexpression of Lac I repressor.
	A convenient MCS allows flexible and easy cloning of the insert.
	Start codon is provided by an Nde I site.
	Inserts can be expressed as C-terminal tagged His6 fusion proteins for easy purification of full length proteins.
	RGS motive prior His tag allows detection and/or immunoprecipitation of the expressed protein with commercial anti-RGS antibodies.
	(Comes with 500 pmol of rev & fw primer each.)

Article	Quantity	Catalog No.	Price
pEG-His1	20µg	V001 0020	EURO 179.00





  Western blot of whole cell lysates 
  from 5 E. coli clones 
  expressing the toxic protein EBNA2.
  (ni = not induced, i = induced)
  

GLOW  Microscopy Mounting Medium

GLOW is a mounting medium for immunofluorescence microscopy. Its new formula stabilizes fluorochromes and substantially decreases bleaching under UV radiation. This results in prolonged gleaming signals and higher contrast of the images. Another innovation of GLOW is its property to gently solidify after the attachment of the cover slide. This feature a) conserves the cells in their permanent appearance and b) permanently fixes the cover slide. Thus the use of "nail polish" for slide immobilisation is no longer necessary. These benefits make GLOW the tool of choice to get the finest images from your precious immuno-fluorescences. For your convenience GLOW comes in an easy-to-use pipet flask.

Article	Quantity	Catalog No.	Price
GLOW	10 ml	M001 0010	EURO 79.00





  Confocal images of FITC stained cells
  after 0 & 3 mins with & without GLOW (no MM).
  

T.Wax  HotStart PCR Beads

T.Wax is a biologically inert wax with a melting point between 69-73°C. It comes in ready-to-use beads of 20 mg (30 µl) to allow a contamination-free and easy handling. By using T.Wax instead of mineral oil it is possible to apply an easy and reproducible protocol for hot-start PCR. In the first step all PCR components except for Taq-polymerase are provided with a T.Wax bead, briefly heated to 95°C and then cooled to room temperature. The wax now forms a solid barrier on top of the reaction mixture. In the second step the Taq-polymerase is added on top of this barrier. During the first denaturation step in the cycler the wax melts again and allows the enzyme to fall through the liquid wax. That way the first contact of the Taq-polymerase and the other reaction components takes place at 95°C, preventing misannealing and the amplification of unwanted fragments. After the amplification the wax barrier can easily be pierced with a pipet tip to remove the reaction mix without oil contaminations. T.Wax beads are suitable for 200 µl and 500 µl reaction tubes and a reaction volume of up to 100 µl. Of course T.Wax is absolutely DNA- and RNA-free!

Article	Quantity	Catalog No.	Price
T.Wax	500 beads	P001 0500	EURO 89.00


Literature:
Bassam, et al. 1993. Automated Hot Start PCR using minerat oil and paraffin wax. BioTechniques. 14(1):30-34.